This will be the fourth DFB Cup tie between the two Bundesliga rivals. The previous meeting was in the quarter-finals of the 2019/20 season with the Werkself Women winning 3-2 after extra time.
Bayer 04 beat second division RB Leipzig 3-1 in the second round to qualify for the Round of 16; TSG went through to the next round with a 5-0 win at Regional League FC Würzburger Kickers.
The Round of 16 ties will be played on 30 and 31 October. Sky will broadcast a top game in each round.
Turbine Potsdam vs FC Köln
SC Freiburg vs VfL Wolfsburg
Bayer 04 vs TSG Hoffenheim
Bayern Munich vs Eintracht Frankfurt
Werder Bremen vs SC Sand
Karlsruher SC vs FC Carl Zeiss Jena
Sportfreunde Siegen vs SV Henstedt-Ulzburg
Hamburg SV vs SGS Essen
An important victory in an emotional, hard-fought away match: Bayer 04 claimed a 4-3 win at 1. FSV Mainz 05 on Bundesliga Matchday 7. Alejandro Grimaldo with a brace and Christian Kofane scored for the Werkself in an impressive first half, with Martin Terrier also getting on the scoresheet late in the second half on his comeback after nine months out injured.
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Kasper Hjulmand managed 1. FSV Mainz 05 during the 2014/15 season. Now he returns to the MEWA ARENA as head coach of Bayer 04 Leverkusen on Saturday 18 October (kick-off 15:30 CEST). The record against the 05ers as well as the hosts' underwhelming start to the season favour the Werkself, but other stats and comments reveal a battling Mainz side who are determined to get back on track. The Matchday News brings you the latest ahead of the Bundesliga Matchday 7 clash.
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Bayer 04 Women have less than 72 hours to recover from the rearranged derby against Köln. Coach Roberto Pätzold's side host VfL Wolfsburg, who are level on points, in a crunch match at the Ulrich-Haberland-Stadion on Sunday (kick-off 17:15 CEST).
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Bayer 04 return from the international break with a trip to Mainz in the Bundesliga on Saturday. Ahead of the Matchday 7 fixture, head coach Kasper Hjulmand spoke at his press conference on Friday about the 05ers, returning to his former club and provided a fitness update on the squad.
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