This will be the fourth DFB Cup tie between the two Bundesliga rivals. The previous meeting was in the quarter-finals of the 2019/20 season with the Werkself Women winning 3-2 after extra time.
Bayer 04 beat second division RB Leipzig 3-1 in the second round to qualify for the Round of 16; TSG went through to the next round with a 5-0 win at Regional League FC Würzburger Kickers.
The Round of 16 ties will be played on 30 and 31 October. Sky will broadcast a top game in each round.
Turbine Potsdam vs FC Köln
SC Freiburg vs VfL Wolfsburg
Bayer 04 vs TSG Hoffenheim
Bayern Munich vs Eintracht Frankfurt
Werder Bremen vs SC Sand
Karlsruher SC vs FC Carl Zeiss Jena
Sportfreunde Siegen vs SV Henstedt-Ulzburg
Hamburg SV vs SGS Essen
Bayer 04 have signed left-back Carolin Simon from champions Bayern Munich for the coming season. The 33-year-old former Germany international, who played for Leverkusen from 2013 to 2016, has signed a contract to 30 June 2028.
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Bayer 04 Women have strengthened for the coming season with the signing of Lobke Loone. The 23-year-old Dutch striker joins from Utrecht on a contract until 30 June 2028.
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The Werkself returned from the near one-month break in the Google Pixel Women's Bundesliga with a convincing victory at home. Roberto Pätzold's side beat SC Freiburg 4-1 on Sunday afternoon, with goals from Katharina Piljic, Estrella Merino Gonzalez, Cornelia Kramer and Carlotta Wamser ensuring a 13th win of the season at the Ulrich-Haberland-Stadion. The Black and Reds, who are now on 40 points, move up to fourth place at least overnight.
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The fifth consecutive derby win over 1. FC Köln was a hugely significant one in terms of the battle for the UEFA Champions League places. Goalkeeper Janis Blaswich, speaking for the whole team, dedicated the 2-1 victory in the cathedral city to the travelling support: "I'm especially happy about this victory for our fans!" Get the rest of the reaction from those involved and the media, as well as some impressive stats on two-goal hero Patrik Schick and a look at the all-time Bundesliga table, in the Werkself Review.
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